Ex VivoandIn SituEvaluation of ‘Dispelling-Wind’ Chinese Medicine Herb–Drugs on Intestinal Absorption of Chlorogenic Acid

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Abstract

This study aims to investigate the additive or synergistic effects and mechanism of intestinal absorption of extracts from two commonly used ‘dispelling-wind’ TCM botanical drugs [roots ofAngelica dahurica(Hoffm.) Benth. & Hook. f. ex Franch. & Sav. (RAD) andSaposhnikovia divaricata(Turcz.) Schischk. (RSD)] using chlorogenic acid as a marker substance.Ex vivoeverted intestinal sac andin situsingle pass perfusion methods using rats were employed to investigate the effects of two TCM botanical drugs extracts on the intestinal absorption of chlorogenic acid. Both the extracts of RAD and RSD showed synergistic properties on the intestinal absorption of chlorogenic acid. The verapamil (a P-gp inhibitor) and intestinal dysbacteriosis model induced by norfloxacin increased the Papp and Ka of intestinal absorption of chlorogenic acid. These synergistic effects on intestinal absorption in a rat model can be correlated with the inhibition of P-gp and regulation of gut microbiota. This experimental approach has helped to better understand changes in the absorption of chlorogenic acid under different conditions. Copyright © 2015 John Wiley & Sons, Ltd.

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