Polygonum aviculareL. is a member of the Polygonaceae family of plants, which has been known for its antioxidant and anti-obesity effects. However, the wound healing function ofP.aviculareextract has not been assessed. In this study, we identified a novel property ofP.aviculareextract as a Wnt/β-catenin pathway activator based on a screen of 350 plant extracts using HEK293-TOP cells retaining the Wnt/β-catenin signaling reporter gene.P.aviculareextract accelerated the migration of HaCaT keratinocytes without showing significant cytotoxicity. Moreover,P.aviculareextract efficiently re-epithelized wounds generated on mice. Additionally, ingredients ofP.aviculareextract, such as quercitrin hydrate, caffeic acid, and rutin, also accelerated the motility of HaCaT keratinocytes with the activation of Wnt/β-catenin signaling. Therefore, based on our findings,P.aviculareextract and its active ingredients could be potential therapeutic agents for wound healing. Copyright © 2016 John Wiley & Sons, Ltd.