A protocol for transformation and regeneration of Antirrhinum majus

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Abstract

Summary

A transformation and regeneration protocol for Antirrhinum majus that allows recovery of transgenic plants within seven months is described. Four different lines transformed with three different constructs have been tested for transformation frequency and regeneration capacity. Regenerated plants were phenotypically indistinguishable from wild-type plants and a GUS reporter gene under the control of the CaMV 35S promoter could be expressed in all tested organs of the plant, including leaves, roots and floral organs. The transgene was heritable through both male and female gametes.

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