Photorespiratory metabolism is essential in all oxygenic photosynthetic organisms. In plants, it is a highly compartmentalized pathway that involves chloroplasts, peroxisomes, mitochondria and the cytoplasm. The metabolic pathway itself is well characterized, and the enzymes required for its function have been identified. However, very little information is available on the transport proteins that catalyze the high metabolic flux between the involved compartments. Here we show that theA BOUT DE SOUFFLE(BOU) gene, which encodes a mitochondrial carrier, is involved in photorespiration in Arabidopsis.BOUwas found to be co-expressed with photorespiratory genes in leaf tissues. The knockout mutantbou-2showed the hallmarks of a photorespiratory growth phenotype, an elevated CO2 compensation point, and excessive accumulation of glycine. Furthermore, degradation of the P-protein, a subunit of glycine decarboxylase, was demonstrated forbou-2, and is reflected in strongly reduced glycine decarboxylase activity. The photorespiration defect inbou-2has dramatic consequences early in the seedling stage, which are highlighted by transcriptome studies. Inbou-2seedlings, as inshm1,another photorespiratory mutant, the shoot apical meristem organization is severely compromised. Cell divisions are arrested, leading to growth arrest at ambient CO2. Although the specific substrate for the BOU transporter protein remains elusive, we show that it is essential for the function of the photorespiratory metabolism. We hypothesize that BOU function is linked with glycine decarboxylase activity, and is required for normal apical meristems functioning in seedlings.