The Arabidopsis LecRK-VI.2 associates with the pattern-recognition receptor FLS2 and primesNicotiana benthamianapattern-triggered immunity

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Abstract

SUMMARY

Pattern-triggered immunity (PTI) is broad spectrum and manipulation of PTI is believed to represent an attractive way to engineer plants with broad-spectrum disease resistance. PTI is activated upon perception of microbe-associated molecular patterns (MAMPs) by pattern-recognition receptors (PRRs). We have recently demonstrated that the L-type lectin receptor kinase-VI.2 (LecRK-VI.2) positively regulatesArabidopsis thalianaPTI. Here we show throughin vitropull-down, bimolecular fluorescence complementation and co-immunoprecipitation analyses that LecRK-VI.2 associates with the PRR FLS2. We also demonstrated that LecRK-VI.2 from the cruciferous plant Arabidopsis remains functional after interfamily transfer to the Solanaceous plantNicotiana benthamiana. Wild tobacco plants ectopically expressingLecRK-VI.2were indeed more resistant to virulent hemi-biotrophic and necrotrophic bacteria, but not to the fungal pathogenBotrytis cinereasuggesting that, as with Arabidopsis, theLecRK-VI.2protective effect inN. benthamianais bacteria specific. Ectopic expression ofLecRK-VI.2inN. benthamianaprimed PTI-mediated reactive oxygen species production, mitogen-activated protein kinase (MAPK) activity, callose deposition and gene expression upon treatment with the MAMP flagellin. Our findings identified LecRK-VI.2 as a member of the FLS2 receptor complex and suggest that heterologous expression of components of PRR complexes can be used as tools to engineer plant disease resistance to bacteria.

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