The Arabidopsis LecRK-VI.2 associates with the pattern-recognition receptor FLS2 and primesNicotiana benthamianapattern-triggered immunity

    loading  Checking for direct PDF access through Ovid


SUMMARYPattern-triggered immunity (PTI) is broad spectrum and manipulation of PTI is believed to represent an attractive way to engineer plants with broad-spectrum disease resistance. PTI is activated upon perception of microbe-associated molecular patterns (MAMPs) by pattern-recognition receptors (PRRs). We have recently demonstrated that the L-type lectin receptor kinase-VI.2 (LecRK-VI.2) positively regulatesArabidopsis thalianaPTI. Here we show throughin vitropull-down, bimolecular fluorescence complementation and co-immunoprecipitation analyses that LecRK-VI.2 associates with the PRR FLS2. We also demonstrated that LecRK-VI.2 from the cruciferous plant Arabidopsis remains functional after interfamily transfer to the Solanaceous plantNicotiana benthamiana. Wild tobacco plants ectopically expressingLecRK-VI.2were indeed more resistant to virulent hemi-biotrophic and necrotrophic bacteria, but not to the fungal pathogenBotrytis cinereasuggesting that, as with Arabidopsis, theLecRK-VI.2protective effect inN. benthamianais bacteria specific. Ectopic expression ofLecRK-VI.2inN. benthamianaprimed PTI-mediated reactive oxygen species production, mitogen-activated protein kinase (MAPK) activity, callose deposition and gene expression upon treatment with the MAMP flagellin. Our findings identified LecRK-VI.2 as a member of the FLS2 receptor complex and suggest that heterologous expression of components of PRR complexes can be used as tools to engineer plant disease resistance to bacteria.

    loading  Loading Related Articles