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Face and loss of face (LOF) are important social and clinical constructs in many cultures. The present study evaluated the psychometric properties of the LOF Scale in 4 samples of European Americans and Asian Americans with a total of 2,057 participants. We found LOF Scale scores to have high internal reliability across all samples. Confirmatory factor analyses comparing 1- and 2-factor models supported a 1-factor structure for both European and Asian Americans, albeit 4 items (Items 3, 13, 14, and 20) were found to be noninvariant across the 2 groups. Two error covariances between Items 2 and 3, and between Items 11 and 20 were both substantial and invariant across groups. Tests of latent mean differences revealed a mean LOF score that was significantly higher for Asian Americans than for European Americans. Finally, the LOF scores correlated with affective distress and self-construal equally for Asian Americans and European Americans, correlated with some factors in collective self-esteem for both groups, and correlated with acculturation for Asian Americans. These results supported the LOF Scale as a psychometrically sound tool for assessing the unidimensional concept of the LOF across cultures.