Molecular Identity of Uncoupling Proteins in Thermogenic Skunk Cabbage

    loading  Checking for direct PDF access through Ovid

Abstract

Thermogenic skunk cabbage has been reported to have two types of uncoupling protein (UCP), a typical 6-transmembrane (TM) SrUCPA and an atypical 5-TM SrUCPB. To verify further the role of SrUCPs in thermogenic skunk cabbage, we examined the molecular identity of SrUCPs in more detail. Both mRNA and genomic analyses supported the presence of SrUCPA, but not SrUCPB. Furthermore, SrUCP protein purified from spadix mitochondria was identified as SrUCPA by mass spectrometry. These results clearly indicate that SrUCPA is the major expressed UCP in skunk cabbage, and the presence of atypical SrUCPB is unlikely to be associated with thermogenesis of skunk cabbage.

Related Topics

    loading  Loading Related Articles