Rapid Assessment of Primer Combinations and Recovery of AFLP™ Products using Ethidium Bromide Staining

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Abstract

AFLP™ is a novel high-resolution fingerprinting method that can be used to delineate intraspecific relationships among a large variety of fungi and plants. We demonstrate that with the appropriate technical modifications, ethidium bromide staining and non-denaturing polyacryalmide minigels can be an inexpensive and time saving alternative for screening DNA samples for suitable AFLP primer pairs. Furthermore, the recovery of ethidium bromide stained polymorphic DNA fragments is not as tedious as the recovery of isotopic DNA fragments.

Abbreviations: EDTA, ethylene diamine tetraacetic acid; BSA, bovine serum albumin.

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