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Two experiments were conducted to determine the dietary threonine (Thr) requirement of Pekin ducks from hatch to 14 d of age. In experiment 1, practical corn-soybean meal diets were formulated to contain 0.78, 0.84, 0.90, 0.96, and 1.02% Thr (0.74, 0.83, 0.88, 0.92, and 1.00% Thr on an analyzed basis). In experiment 2, corn-soybean meal diets supplemented with 11 crystalline amino acids were formulated to contain 0.60, 0.70, 0.80, 0.90, 1.00, and 1.10% Thr (0.60, 0.75, 0.89, 0.95, 1.01, and 1.09% Thr on an analyzed basis). In both experiments, diets were fed to 8 replicate cages with 6 male ducks per cage. Body weight and feed intake from each cage were recorded weekly. At 14 d of age, breast meat, ileal digesta, and serum were collected to determine breast meat yield, mucin secretion, and serology parameters. In both studies, the estimated Thr requirement (expressed as % dietary Thr basis) for 14 d BW and BW gain (BWG) by quadratic broken-line (QBL) regression were similar, which were 0.87 and 0.86%, respectively. Additional measures in both experiments resulted in Thr requirements via QBL regression in rank order of crude mucin secretion < breast meat yield < serum immune activity. Summing up the estimates from both studies, the Thr requirement ranged from a low of 0.81% to maximize feed intake (FI) to a high of 1.00% to maximize serum Rb L100 by QBL regression. Correspondingly, the Thr requirement varied between a low of 0.90% to maximize crude mucin secretion on a dry matter intake (DMI) basis and a high of 0.98% to maximize feed-to-gain when using quadratic regression.