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To examine the effect of body weight-supported treadmill training (BWSTT) on gait and gross motor skill development in children (2-5 years old) with developmental delay who are ambulatory.Twenty-four subjects (12 control and 12 BWSTT) were enrolled in this randomized control trial. All subjects continued to receive physical therapy. Subjects were tested at baseline, 4 weeks, 6 weeks, and at 6 weeks after completion of BWSTT. Outcomes were assessed using the 10-m walk test and Gross Motor Function Measure-D and E.Significant improvements were seen in gait velocity and gross motor skill attainment. With positive interactions in both the 10-m walk test and Gross Motor Function Measure-E, the BWSTT group as compared with the control group demonstrated functional gains in gait velocity and gross motor skills, P = .033 and .017, respectively.A 6-week high-intensity BWSTT program can improve gait velocity and influence functional gains.