Design and engineering of synthetic binding proteins using non-antibody scaffolds
Harnessing design to probe the essential Hsp90 protein interaction network in vivo
The protein design program MUMBO and its application for the design of discriminative heterodimeric proteins
Evolution and assembly of protein complexes
Molding the plasma membrane at sites of endocytosis
Protein-protein interactions in vesicle transport: structural studies on Rab5 activation, effector/Rab/Arf specificities, and ubiquitin recognition
Regulation of Intracellular Membrane Fusion
Cytoplasmic dynein, FYVE protein SARA, and PI3P-regulated vesicular targeting of rhodopsin underlies formation of the light sensing organelle in mammalian rods
COPII coat assembly and vesicle budding on synthetic membranes
Using proteins of reduced complexity to understand cooperativity in folding
Membrane protein folding: insights into folding transition states
Creating Molecular Switches by Alternate Frame Protein Folding
Molecular mechanism of fibrinogen as a novel extracellular chaperon-like protein
Signal amplification driven by an actin feedback loop
Hierarchical Regulation of WASP/WAVE Proteins
Chemical biology of Cell Division
Actin dynamics powers viral transport to the nucleus and cell surface
Single molecule protein kinetics
A New Spin for Protein Dynamics
Structural biology and fragment-based approaches to lead discovery
Protein Kinases: Dynamic Targets for Drug Design
Design of Potent D-peptide Inhibitors of HIV-1 Entry
Probing RNA sequence specificity and function of PUF proteins
Quality control on the ribosome following peptide bond formation
Structural Reorganization of α-Synuclein Under Different Sequence and Environmental Conditions Observed by NMR and Molecular Simulations: Implications for Aggregation
Promiscuous proteins: folding and interactions of intrinsically disordered proteins
Amyloid fibril growth visualized by thioflavin T fluorescence and probed by H/D exchange
Structure and interactions of a disordered malaria surface antigen
Mechanisms of biological regulation via dynamic complexes involving intrinsically disordered proteins
HIV-1 Integrase Inhibitors: From the Bench to the Clinic and Back Again
Structural and functional studies of the HIV-1 restriction factor APOBEC3G
Diversity-Generating Retroelements
Dynamics of Orotidine Monophosphate Decarboxylase Catalysis: A Simple Reaction in a Complex Environment
Charges in the hydrophobic core of proteins: the motif essential for energy transduction
Seeing the Invisible by Solution NMR
Methodological advances in computer simulation of biomolecular systems
Single molecule fluorescence and atomic force microscopy studies of DNA mismatch repair and DNA damaged-induced apoptosis
Synergistic interactions between components of the synaptic vesicle fusion machinery
Crystal structure of synaptic adhesion complex; insights into the molecular assembly at the synaptic membrane
The Role of Dynamin Family Members in Membrane Fission
Viral Capsid Assembly and Maturation
Molecular machines that assemble biological membranes
Regulatory networks for cell-fate choice in the immune system
Homo-oligomerization as a common way for Proteins to modulate their activities
Interactome Networks and Human Disease
Signal transfer from receptor to G-protein: the rhodopsin-transducin model
The molecular principles of gamma delta T cell recognition of ligand: complex or simple?
Structural basis of Shh antagonism by Hedgehog-Interacting Protein
Protein signaling and motion: a joint protein-ligand perspective of Pin1
Understanding cytokine receptor signaling
Computational Integration of Diverse Structural and Kinetic Data for Visualizing Macromolecular Assemblies and Processes
The structure of the tail-anchored targeting protein Get3
Alternating access mechanism of a bacterial sodiumcoupled aspartate transporter
Insertion of membrane proteins into the endoplasmic reticulum
Teaching Old Enzymes New Tricks
Protein Homeostasis in Health and Disease
Allosteric Regulation of Protein Kinases
NMR Portrait of Protein Kinase A: Unveiling Allostery and Cooperativity
Bacterial Quorum Sensing
Order-disorder allosteric transition to regulate receptor binding in signaling
Conformational Dynamics, Structure & Mechanism of Hsp90 Chaperones
Ultrasonication-dependent production and breakdown lead to monodispersed and minimum-sized amyloid fibrils
Do amyloid oligomers act as traps for misfolded proteins?
The role of poly amines in modulating amyloid-induced neurotoxicity in Alzheimer's disease
A molecular probe for rapid in situ amyloid aggregation detection
Charge neutralization and collapse of the C-terminal tail of alpha-synuclein at low pH
Elucidation of Neurotoxicity Inhibitors Binding Sites on beta-Amyloid Aggregates, as Means to Explore Residues That Are Significant For Toxicity
Dissolving Alzheimer's Amyloid Plaques with Red Wine: Insights from Molecular Dynamics Simulations
Structure and Dynamics of Non-Fibrillar Oligomers of PrP(106-126)
Computational studies of α-synuclein protein folding and assembly in Parkinson's disease
Disulfide-reduced ALS-variants of copper zinc superoxide dismutase are partially denatured
Delineating the conformational elements responsible for ligand induced oligomerization of [beta]-2 microglobulin
Misfolding of the DNA binding domain in tetrameric p53: Effects of oligomerization
MIST: Maximum Information Spanning Trees for Dimension Reduction of Biological Data Sets
Putative Prolyl Aminopeptidases in Candida albicans genome.
Hepatic protein abundance patterns during schistosome-mediated liver disease
Cloning of a metal transporter from malaria mosquito Anopheles gambiae tissue cDNA library
A Lot More Than Coordinates: A Short Tour of a PDB file
NMR Data for Proteins in Motion at BioMagResBank (BMRB: )
Structural bioinformatics mutation analysis reveals genotype-phenotype correlations and suggests molecular mechanisms in VHL disease
Purification and Structural Characterization of a Mutant Wheat Metallothionein
Purification of VLP-based vaccines using a new prepacked gel filtration column
Rapid screening of antibody aggregates and protein impurities using short gel filtration columns
Method for rapid purification, modification and immobilization of recombinant protein
Purification, kinetic characterization, and structure based analysis of Janus Tyrosine Kinase isoforms
Purification of ADAM8 and characterization of its auto-activation mechanism
Affinity Capture of Plasma Proteins Using Heparin HyperD® M
Folding free-energy landscape and kinetics of villin headpiece HP35 from Markovian milestoning
Free energy calculation and decomposition of HIV-1 protease-Darunavir binding by MM-PB/GBSA and Thermodynamic integration method
Using Molecular Dynamics to Investigate Substrate Recognition and Co-evolution in HIV-1 Protease
Identifying and removing sources of error in cluster-expansion energy models for protein design
Regulating Conformation of Prion Protein through Ligand Binding
NMR Structure Determination by Conformational Space Annealing
Simulation of a membrane protein tertiary complex: Study of the interaction interface and conformational dynamics of the precoupled complex between rhodopsin and transducin
Reliable prediction of protein interactions by projecting PDB on novel genomes
The role of flexibility in RNA recognition and binding in the tandem zinc finger domain of TIS11d
Potential Aggregation Prone Regions in Monoclonal Antibodies and Their Contribution towards Antigen Recognition
New evidence for metal-dependent activation of E. coli RNase I
Examining the Roles That Individual Residues of Glutamine-Containing Substrates Play in Factor XIIIa Specificity
Recombinant Expression and Purification of Dehydratase Domains from the Enzymatic Complex Responsible for the Biosynthesis of Omega-3 Fatty Acids in Marine Bacteria
Characterization of an aldolase/dehydrogenase complex in the polychlorinated biphenyl degradation pathway
Influence of conserved residues on individual steps of protein splicing of the non-canonical Pyrococcus abyssi PolII intein
Development of protein purification strategies using the Pyrococcus abyssi PolII intein
Enzyme dynamics involved in substrate specificity: Evidence for differential conformational changes distant from the active site during the aldolase catalyzed reaction
New Insights Into Class II HMG-CoA Reductase Mechanism
Oligo(Ethylene Glycol) Monolayer Properties That Correlate with Protein Fouling Resistance
A Dark to Bright Fluorescent Reporter of Proteolytic Activity
Immunofluorescence Detection Applications Using DyLight(TM) Fluorescent Conjugates
Mechanisms that control the stress-induced accumulation of the chaperone hsc70 in nucleoli
Sorting in early endosomes: connections to dockingand fusion-associated factors
Mechanistic investigation of SNARE-mediated fusion of large liposomes
Overexpression, Purification and Biophysical Characterization of Human Interleukin 1alpha
Understanding the Role of Ankyrin domain of the 43-kDa subunit of the Chloroplast Signal Recognition Particle in Protein Targeting
Interaction of brefeldin A-inhibited guanine nucleotide-exchange protein (BIG) 1 and kinesin motor protein KIF21A
Membrane Associated Structure for the C2 Domains of Synaptotagmin 1: Evidence for Bilayer Bridging
Systematic Analysis of Side Chain Effects on the Interhelical Packing of a CFTR Helical Hairpin
structural stability and functional remodeling of human HDL: Role of particle size, protein composition and oxidation
Insight into key residues involved in oligomerization and drug interactions of HIV-1 Vpu
Microstructure and phase behavior of protein-detergent complexes leading to crystallization of photosynthetic reaction center
Total Segment Hydrophobicity Modulates the Effects of Polar Residue Substitutions in Transmembrane Segments
Towards understanding membrane protein sorting: computational and experimental comparisons of alpha helices from globular and transmembrane proteins.
The sequence of an extracellular loop of CFTR dictates the packing of adjacent transmembrane helices
Overexpression and purification of a disulfide containing membrane protein
The tail sheath structure of bacteriophage T4: a molecular machine for infecting bacteria
Structural evidence for common ancestry of the nuclear pore complex and vesicle coats
Characterization of myosin-actin interface of molluskan striated muscles
The DnaX complex chaperones DNA polymerase III to the initiation complex
The interactions of human translation initiation factor eIF5B: insights into the coordination between start codon selection and ribosomal subunit joining
Homodimer formation of the cytosolic domain of fission protein Fis1 from S. cerevisiae is kinetically trapped
Effective translation of the PH084 mRNA on ribosomes needs Rpll2bp
Unidirectional motion observed by an in silico single molecule experiment of an actomyosin system
Structure-based analysis reveals hydration changes of Lysozyme and Glucose isomerase induced by arginine hydrochloride
Protein Structural Comparison with Qualitative and Quantitative Assessment
Variable Pathlength Spectroscopy for The Determination Of Protein Extinction Spectra and Protein Quantitation
Applications of the SNAP-tagTM Protein Labeling Technology
High-throughput determination of protein stability, ligand binding affinity, and catalytic activity
A switch in the glycosylation site of RAGE is promoted by the G82S polymorphism
Development of Chemical Methods for the Detection of Protein N-Homocysteinylation
Microbial Glycosylation: The Outer Surface of the Archaeon Methanosarcina mazei
Development of Chemical Methods for the Analysis of Protein Post-Translational Modifications (PTM's)
Phosphorylation-Dependent Modulation of Intramolecular Domain Association Regulates Discs Large Function
Introducing mandelate dehydrogenase activity based on rational design technique into the bs LDH
Use of D-amino acids to stabilize protein motifs
Designing Cyanovirin-N Derivatives for Stability and Combinatorial Screening
Expression of Recombinant Human Enterokinase Light Chain in Kluyveromyces Lactis
Computational re-engineering of organophosphorus hydrolase for enhanced enzymatic activity and stability using transition state theory
Photocontrol of Rho GTPase activation by leveraging of a small-molecule modification
Engineering of single immunoglobulin domain of ICAM-1 for its recognition of rhinovirus and integrin
A pair of similar designed Cro protein sequences that mimic an evolutionary secondary switching event
Engineering E. coli DNA polymerase III alpha for translesion synthesis
Cyanylated cysteine is an infrared probe of order-disorder transitions in transiently helical proteins
Cooperatively folded and marginally stable: A new state in the disordered protein universe
Stabilization of a Disordered Aldolase Variant that Causes Hereditary Fructose Intolerance with Small Zwitterionic Osmolytes
Uncovering Order in Disorder: Characterizing the Structural Heterogeneity of an Elastin-like Peptide
Interactions of the tau microtubule-binding domain with anionic lipid surfaces
Probing Slow Dynamics by NMR spectroscopy in high molecular weight proteins: Human Prolyl Oligopeptidase
Using Molecular Modeling to Investigate the Curious Nature of the p53 C-terminus
Evidence for Partially Unfolded States in a Triple-Helical Model Peptide Containing the Collagenase Cleavage Site
Ionic strength effect in the binding mechanism of an SH3 domain
Investigating Factor XIII Structural Changes in the Presence of Mono and Divalent Cations Utilizing HDX coupled with MALDI-TOF-MS
Nuclear Magnetic Resonance (NMR) Studies of NADH Oxidase (NOX)
Binding Cooperativity from an Induced Folding Event in Aminoglycoside N6'-Acetyltransferase-Ii
Dynamics Analysis of Unstructured FG-Nucleoporins
Drug binding and mutation decouple global dynamics within E. coli dihydrofolate reductase.
Dynamic activation of a transcription factor
The importance of conserved hydrophobic core residues on the structure, stability and folding of Fas-associated death domain protein
Interpretation of p-CyanoPhenylalanine Protein Fluorescence in Terms of Solvent Exposure and Contributions from Side Chain Quenching
NMR Characterization of Villin Headpiece Helical Subdomain in 8M Urea Reveals the Presence of Secondary Structure in the Denatured State
Experimental and computational evaluation of the role of van der Waals and electrostatic interaction in driving assocation of transmembrane helices
Extended monomeric intermediate upon cold denaturation of the dimeric phosphofructokinase-2 from E. coli
Role of Proline in the Folding of Conotoxins
Effect of Osmolytes on Protein Stability and Folding
A split ubiquitin split tetra-Cys sensor for monitoring in vivo folding
A simple algorithm for numerical Laplace inversion of kinetic data yields insight into unfolding mechanisms in a heterogeneous, posttranslationally-modified SOD1 population
Folding of the PBX Homeodomain Probed by DSC and NMR Spectroscopy
The folding of PCP (Pyrrolidone Carboxyl Peptidase) proceeds in the denatured state similar to the stage of molten globule
Gly192 at hinge 2 site in the chaperonin GroEL plays a pivotal role in the dynamic apical domain movement that leads to GroES binding and efficient encapsulation of substrate proteins
Structural analysis of an off-pathway folding intermediate in SpoOF, a CheY-like protein, by HX-MS and mutagenesis
Unzip protein coiled coils by force
Monitoring the folding of the p53 core domain by FlAsH fluorescence
Folding and Association of ALS-Linked Superoxide Dismutase Variants
A peptide recognition event leading to the native fold of E. coli thioredoxin: an NMR study
The role of a conserved array of phenylalanines in the folding of the intracellular lipid-binding protein, cellular retinoic acid-binding protein
Protein folding: Independent unrelated pathways or predetermined pathway with optional errors
Opposite allosteric mechanisms in CAP and TetR
Phosphorylation functions with aromatic residues to modulate the autoinhibition of Ets-1 DNA binding
Probing the Mechanism of IAPP Amyloid Assembly using Structural Peptidomimetics
Association reaction between IAPP fibrils and lipid vesicles
Small molecules and macromolecules for the inhibition of β2 microglobulin fibrillogenesis
Designing a Protein Based Inhibitor of Amyloid Beta Fibrils
Self Assembly of Coiled-Coil Peptide Porphyrin Complexes
Mass Spectrometry-based Amide Exchange in Optimizing Protein Constructs for NMR Structure Determination
Elucidating the chemical and physical properties controlling protein expression and crystallization
Protein dynamics in the catalytic reaction cycle of the oxidative DNA repair enzyme AlkB
Structural Biology of Influenza Viruses - Applications of Technologies from Structural Genomics
Analysis of active sties and ligand-binding sites for the prediction of detailed enzyme functions
In Silico Studies on Hemagglutinin Protein of H5N1 Subtype
The PSI SGKB - a one-stop shop for biologists interested in protein structure, function, sequence, methods, and more
Proteomic and Bioinformatic Analysis of the alpha7 Nicotinic Receptor Complex in Brain
Cleavable Biotinylated Probes
Evolution of chromate resistance determinants
The effect of gene optimization and correlation of cell-free and cellular protein expression: a large-scale study
30X faster protein HPLC analysis in ion-exchange mode
Simple immunoprecipitation using paramagnetic beads
Strategies to express and purify HIV-1 RT
Purification of DNA-tethered HIV-1 RT to understand the mechanism of action of a novel nucleotide analog inhibitor GS-9148 Diphosphate.
A New Paradigm in Ni Affinity Purification
The Gelsolin Superfamily - Activation and Function
Moonlighting Functions for the Glycolytic Enzyme Aldolase: Implications for Cytoskeleton Dynamics
Dynein Light Intermediate Chain 1 is Required for Cell Cycle Progression through the Metaphase Spindle Assembly Checkpoint
The assembly motif of a bacterial small multidrug resistance protein
Covalently bound thiocyanate as a localized probe of membrane-protein interactions
Detergent binding explains anomalous SDS-PAGE migration of membrane proteins
Potential of neural cell adhesion molecule as differentiation marker in Ashwagandha extract treated C6 glioma cells
Ornithine decarboxylase antizyme inhibitor prevents cellular insults-induced apoptosis
Analysis of the effect of mutations of the conserved arginine residue located in the middle of the membrane-spanning domain of the HIV-1 envelope protein subunit gp41
Rational Design of a Fusion Partner for Membrane Protein Over-Expression in E. coli
Monitoring membrane protein unfolding by pulse proteolysis
CED-9 and mitochondrial homeostasis in C. elegans muscle
Understanding Specificity Determinants of Interactions between Bcl-2 Family Proteins using Yeast Surface Display Screening
Engineering Enzymes to Self-Assemble into Hydrogels
Rational mutagenesis of a cis-specific PPIase based on the structure and reaction scheme of Pin1
Towards a general dehydrogenase enzymatic platform: Engineering an alcohol dehydrogenase for self-assembly and for activity with alternative substrates
Designed Ankyrin Repeat Proteins as Scaffolds for Multivalent Recognition
The effect of the C8-HSL in the folding of the quorum sensing transcriptional regulator SdiA
Structure of a functional box H/ACA RNP bound to a substrate RNA
E.coli DnaB-DnaC Complex with Nucleotide Cofactors: An Allosteric Control of Recognition Process in DNA Replication
A Protein in the Playground: Sliding, Hopping and Swinging of Homeodomain along DNA
The role of conformational flexibility in proteins’ search for their recognition sites
Structure of ERA in Complex with the 3’ End of 16S rRNA: Implications for Ribosome Biogenesis
Quantitative proteomic analysis of ribosome assembly and turnover in vivo
Qualification of Biophysical Methods for a Protein Therapeutic and Application of Qualification Parameters to Comparability Data
A Thermodynamic Perspective on Structure Prediction with Rosetta
Structural classification and modeling of antibody hypervariable loops: knowledge-based approaches
A Histidine Switch in Hemagglutinin-Neuraminidase Triggers Paramyxovirus-Cell Membrane Fusion
Peroxynitrite used to Map Solvent Accessibility of Amino Acids within Proteins
Simulation of MaSp1 protein structure as the main component of Dragline Silk in Latrodectus hesperus
Efficient Enumeration of Protein Secondary Structure Assemblies
Modeling transmembrane helix length explains the role of juxtamembrane residues in PIV5 viral fusion
Replication Protein A structural changes that are associated with binding of single stranded DNA and with Zn ions.
Structural and Biochemical studies of rpfF in DSF biosynthesis and Quorum sensing
Direct Utilization of NMR Chemical Shift Restraints in Protein Structure Determination
Monitoring Nucleotide Binding to E. coli Aspartate Transcarbamoylase by Fluorescence from a Genetically Encoded Unnatural Amino Acid
Understanding the Role of Pin1 in the Processing Fate of Amyloid Precursor Protein
LFG: A Unique Apoptosis Regulatory Protein Family
Elucidation of a pH-folding switch in the Pseudomonas syringae effector protein AvrPto
Mechanism of pH-dependent Activation of Furin
Structural and Biochemical Insights into the Co-evolution of the p1-p6 Cleavage Site with the Nelfinavir-Resistant D30N/N88D HIV-1 Protease
How Hydrogen Bond Redundancy Affects Protein Flexibility
A Pharmacological Chaperone, DGJ stabilizes α-galactosidase (A Fabry Disease Lysosomal Enzyme)
Rosetta Correctly Predicts Mutant Behavior of AraC
Kinetic and ERP studies of the role of divalent metal cations in E. coli Pfk-2
Phosphorylation Induced Structural and Dynamical Changes in Phospholamban Probed by Solution and Solid-State NMR Spectroscopy
Studies of motion in the caspase-7 L2′ loop
The Evolutionary Role of Tandem ACP Domains in Polyketide Synthases
Trojan peptides: two structures, two functions
Solution-Phase H/D Exchange FT-ICR Mass Spectrometry to Determine Leucine Regulation of α-Isopropylmalate Synthase from Mycobacterium tuberculosis
The crystal structure of Atg8 from S. cerevisiae
The Role of Methionine Residues in Activity and Subunit Communication in Malate Dehydrogenase
Allosteric behavior of monomeric E. coli gamma-glutamylcysteine ligase in the presence of non-substrate analogs
Chimeric and hybrid variants of two Cro proteins with 40% sequence identity but different folds
Allosteric activation of DegS, a stress sensor PDZ protease
Molecular determinants of pKa values of internal ionizable groups in proteins: properties of ion pairs in hydrophobic pockets
Structural and Kinetic Studies of Chimeric β-Lactamases
Maltose-binding protein mutants with increased affinity for maltodextrins
Using FlAsH to probe residual structure in Intrinsically Disordered Proteins
A “Control Workgroup” of 24 PSI Protein Targets Used to Test Platform Changes
Determinants of Differential Binding and Cleavage of Kunitz Inhibitors APPI and BPTI by Mesotrypsin
Engineered active integrin (LFA-1) coupled liposomes for the delivery of therapeutic and diagnostic agents to the inflamed endothelium
Crystal Structure of the Trimeric Complex of Interleukin-13, IL-13 Receptor al and the Binding Domain of the Inhibitory Antibody Fab13.2: Implications for the Humanization of Murine 13.2 Anti-hIL-13 Monoclonal Antibody
Immunoproteomics of Streptococcus pneumoniae infection using recombinant protein microarray
Mapping Antibody Epitopes
A protease-resistant immunotoxin against CD22 with greatly increased activity against CLL and diminished animal toxicity
Discovery of activation-specific, metal-ion dependent antibodies against Mac-1 integrin I domain
Toward systemic delivery of therapeutic and diagnostic nanoparticles into the brain via transcytosis-triggering molecular interactions
The protein complex network of a minimal molecular machinery
The Parkin-UbcH8 Ubiquitination Complex: Binding Affinity, Interface Mapping and Implications in Autosomal Recessive Juvenile Parkinsonism
Intrinsically unstructured C-terminal regions of transmembrane PDZ ligands play an important role in clustering scaffold proteins
Biochemical and evolutionary studies of dimerization specificity in histidine kinases
The Role of Electrostatics in the Interaction of EHD1 and Rabenosyn-5
Screening of Small Molecule Inhibitor Targeting the FAK / Paxillin Interaction
Requirements for complex formation in vitro between C-Jun N-terminal Kinase (JNK) and Glutathione-S-Transferase pi (GSTpi)
Understanding Specific Carbohydrate Recognition by the Antiviral Lectin Cyanovirin-N
Characterization of Protein Agglomeration Dynamics Through the Use of Multi-Wavelength Angle-Resolved SPRi
Biophysical characterization of a zinc-based adhesion module in Staphylococcal biofilms
A Synthetic Regulatory System for Exploring Mechanisms of Ultrasensitivity
Pathway of ligand release in visual pigments
Dissecting heterotrimeric G-protein function with energetic component analysis.
Domain 4 of the anthrax protective antigen maintains structure and binding to the receptor CMG2 at low pH
X-ray crystallographic evidence for conformational multiplicity of an HLA-B*2705-bound self-peptide
Investigation of coiled-coil interactions between components of the spindle pole body
Percolation in biology: multivalent protein-protein interaction
Binding analysis and characterization of the NMDA NR2D ionotropic glutamate receptor
Understanding the effectiveness of synthetic crowding agents in stimulating the intercellular environment
Structural Basis for Multimeric Heme Complexation through a Specific Protein-Heme Interaction
Vibrational studies of protein-ligand interactions: Insights into drug binding and selectivity
How Insulin Binds to the Insulin Receptor: A Synthetic Chemical Biology Approach
Characterization of Interleukin 11 agonist & antagonist
Investigating the Source of Ultrasensitivity in the Pins/Gai/Mud Spindle Orientation Pathway
Specificity and promiscuity in heterotrimeric G-protein signal
Characterization of Gtr1/2 small G proteins
Characterization of Inhibitory Anti-IGF-1R Antibodies With Different Epitope Specificity and Ligand Blocking Properties: Implications for Mechanism of Action In Vivo
Expression, purification and functional reconstitution of ligand-gated ion channels 5HT3AR and GABAAR
The Down Regulation of the She Adaptor Protein
Psoralea corylifolia induces spermatogenesis in rats through the activation of cAMP-responsive element modulator (CREM)
Single particle analysis of liposome leakage induced by islet amyloid polypeptide
Sloppy Models, Parameter Uncertainty, and the Role of Experimental Design
An in vitro model for protein translocation using calmodulin binding peptides and Src homology 3 domains
Epigenetic Regulation of Macrophages Response to LPS
Enrichment of Actin and β-Tubulin Interactors by Affinity SNAP-Capture
The fidelity of a molecular RS latch can be improved by cascading signaling pathways
Identification of Differential Kinase Activity by Analysis of Phosphoprotein Sequence Motif Enrichment
Histidine Hydrogen-Deuterium Exchange Mass Spectrometry for Probing the Microenvironment of Individual Histidine Residues in Escherichia coli Dihydrofolate Reductase
Glu272 and Lys212 contribute to Mn2+ binding affinity of Saccharomyces cerevisiae phosphoenol-pyruvate carboxykinase
Non-canonical inteins: Alternate mechanisms of protein splicing
Characterization of Soluble, Deglycosylated BACE1 Isoforms
Characterization of unique enzyme inhibitors as model anti-microbial agents
Biochemical Characterization of a DNA ligase I from Entamoeba histolytica.
Positive cooperativity of the p97 AAA ATPase is critical for the function
The Amyloid Precursor Protein Kunitz Inhibitor Domain is a Highly Specific Substrate of Mesotrypsin
Controlled protein expression by -1 ribosomal frameshifting in vivo
Study of Protein Dynamics in Living Cells using SNAP-tagTM Technologies
Protein Crystallization by in situ Proteolysis
A Rapid Method for Removal of Detergents from Proteins and Protein Digests in a Spin Column Format
ATCG3D Methods for Microfluidic Protein Crystallization
Efficient Refolding, Activation, and Biotinylation of Cathepsin K for Crystallography and Inhibitor Screening
Assessment of Concentration Using a Calibration-Free Method
Analyzing drug resistance in terms of substrate recognition by Hepatitis C Virus NS3 protease
Establishing The Specifics Of Substrate Binding For Cytochrome C Oxidase: A Computer Modeling Approach
Eukaryotic Molecular Chaperones - A Structural Genomics Approach
The Beta-Roll Peptide as a Novel Allosterically-Regulated Scaffold for Biomolecular Recognition
Rationally Dissecting and Enhancing the NKG2D-MICA Immunoreceptor-Ligand Interaction with RosettaDesign
Structural studies of the calcium-dependent conformational behavior of a beta roll peptide
Probing dimer interface surface requirements via internal deletion mutations within the GrpE protein
Targeting transmembrane helices via design
The Study of Myosin and Tropomyosin Reveal General Engineering Principles
Streamlining phage-display library sorting by reversible biotinylation of histidine-tagged proteins
Charge Optimization of Flexible Ligands
Probing Opioid-Induced Glial Activation with Peptide Antagonists
Ubiquitin carboxy hydrolase (UCH-L3): A molecular sensor of protein stability?
Rational design of pH-sensitive Streptococcal protein G mutants by modulation of electrostatic repulsion
Bioinformatic method for protein thermal stabilization by structural entropy optimization
Design of a peptide inhibitor of the viral transcription factor BZLF1
Molecular Recognition Between Enantiomeric Collagen Triple-Helices
Ezβ - an Energy Function for Membrane Insertion of Individual Amino Acids in Transmembrane β-Barrels
Dynamic dissociating homo-oligomers and allosteric regulation
Cyanylated cysteine: a site-specific probe of protein and solvent dynamics
Functional Dynamics of Arginine Kinase
Structural fluctuations of hemoglobin in solution
Probing the Flexibility of Large Conformational Changes in Protein Structures through Local Perturbations
Early signatures of the pathway bifurcation in force-induced unfolding of tubulin monomers
Helix docking dynamics: NMR and MD investigations of a novel Ubiquitin fusion protein
New approaches for modelling motions of functional loops
Characterization of Enzyme Dynamics in an RNase AECP Chimera
Comparison of Flexibility in Antibody Binding Sites as Measured by Frequency Domain Fluorescence Spectroscopy
Protein Elastic Network Models and the Range of Cooperativity
Quasi-harmonic mode description of the protein Boson peak in molecular dynamics simulations
Mapping a partially unfolded state by protein engineering and native-state proteolysis
Identification of structure conserving motions in the protein ubiquitin
Conformational Dynamics of the Enzyme Glutamate Racemase
Probing Protein Tumbling Inside E. coli Cells by In-cell NMR
Crystal and Solution Structures of a Ten-Amino Acid Protein and Its Scale-Free Distribution in Structure Space
Contributions of Aromatic Residues to the Stability of Human γD-Crystallin
Thermodynamics and Cooperativity of Domain Unfolding in Subunits of an Icosahedral Viral Shell: The Delta-Domain of Bacteriophage HK97
Thermodynamic Consequences of Incorporating 4-Substituted Proline Derivatives into a Small Helical Protein
Correlation Between Aldose Reductase and Superoxide Dismutase Inhibition Capacities of Indole-based Analogues of Melatonin Derivatives.
Large Scale Refolding, Purification, and Characterization of BACE and BACE-2 Expressed in E. coli
Dissecting the N-terminal helical domain of Apolipoprotein B
Structures, Basins and Energies in the Protein Coil Library
Effects of Chemical Additives on the Stability and Energy Spectrum of a Model Helical Hairpin Peptide Using Extended Helix Coil Theory (XHC)
Computational and biophysical studies of an ionic network in the tet domain of p53
Structural analysis of partially-folded states in a β/α-repeat protein, CheY, by hydrogen exchange mass spectrometry
Effect of cysteine residues on the self-assembly of VP2 subviral particle of Infectious bursal disease virus
Evidence for both steric zipper and polar zipper structures in polyglutamine fibrillar intermediates
Sensitivity to submicron particle detection with field flow fractionation
Analysis of eukaryote-specific mitochondrial protein networks through data integration and phylogenetic profiling
DNA replication polymerase oligomers are responsible for increased enzymatic activities through a mechanism of dynamic processivity
The mechanism and energetics of DNA supercoil relaxation by human topoisomerase I as revealed through molecular dynamics simulations
The DNA-binding interactions of PhoP from Mycobacterium tuberculosis
Cistanches Herba impairs spermatogenesis through down- regulation of the cyclic AMP response element modulator (CREM) gene expression and protein biosynthesis in mouse testis
A family A DNA polymerase in Entamoeba histolytica
Conformational dynamics in mitochondrial RNA Polymerase
Structural dynamics of the Thermus thermophilus 30S ribosomal subunit induced by a streptomycin-dependence mutation in 16S rRNA
Fast Identification of Functional Family Members Using Fuzzy Functional Forms and Active Site Profiling
Application of a Functional Site-Based Classification Method to the Protein Kinase Superfamily
A Rapid, Robotic, Small-scale Approach to Screening for NMR Structure Determination in the PSI
PSI:Biology - High-Throughput-Enabled Structural Biology
Phosphodiesterase 4B: From Purification to Crystallization
Observation of domain interactions in the protein tyrosine kinase Csk by segmental labeling / NMR
Dissection of the molecular architecture of higher-order assembly of the full-length reelin
Hit Validation by Direct Measurement of Protein Conformational Change
A cell division mutant of ERA in Escherichia coli
The interactions of SET3 complex and its genomewide association map
Critical factors determining dimer formation of human antizyme inhibitor
Probing Hydrophobic Core Sliding as a Possible Mechanism for Enzymatic Activity in HIV-1 Protease
Assessing the Potential Correlation Between Protein Stability and Function
Measurement of Mechanical Property of the Mechano-Sensing Protein p130Cas, and Bioinformatic Analysis of Mechano-Sensing Protein Domains - Possible Mechanical Roles for Unstructured Domains
Structural and Biochemical Insights into Altered Drug Resistance Pathways in Non-Clade B HIV-1 Proteases
Technology Development in the PSI: Highlights from the Center for Eukaryotic Structural Genomics
Tools and Resources Developed at the Joint Center for Structural Genomics, a PSI Production Center
Protein binding residue prediction based on three-dimensional protein modeling
Apoptosis-inducing factor (AIF) induces age-related apoptosis of human gracilis skeletal muscle
The NIPP1:PP1 Holoenzyme
NMR characterization of the FoxE cytochrome, a putative Fe oxidoreductase in a phototrophic iron oxidizing bacterium
gp120 Expression for NMR Studies of the CCR5/gp120/miCD4 Ternary Complex
The Basic Coiled-coil Protein Structures from Human Gut Bacteria Eubacterium eligens and Clostridium symbiosum
Protein Structure Initiative Material Repository (PSI-MR): An open shared public resource for structural genomics plasmids
Preliminary Structure of Orf6: Confirmation of a Thioesterase Hot-dog Fold
Molecular details of the interaction of Protein Kinase A with phospholamban and phospholamban mutants
Chaparral (Larrea tridentata) extracts with haemagglutinating activity exhibits antibacterial properties
Structural characterization of PmrD, which connects the PhoPQ and PmrAB two-component signal-transduction systems
Structural characterization of the complex formed between the XRCC1 N-terminal domain and DNA polymerase beta
The role of palladin/α-actinin in cell motility and invasion
Structural Reorganization of the Anti-Sigma Factor AsiA
Estrogen Receptor Alpha Activation by Calmodulin
Structural basis for diphosphoinositol signaling
Conformational switching of clathrin light chain regulates clathrin lattice formation
Disease-associated mutations in filamin B actin-binding domain decrease structural stability but increase binding affinity to filamentous actin
Structural Genomics of Fe-S Cluster Assembly: Covering a Biosynthetic Pathway
Stretching Single Talin Rod Molecules Activates Vinculin Binding
Calculation of the active-site protonation state for the beta-secretase
Exploring the twin-arginine signal peptide binding site of DmsD
Characterization of the APP, LRP1, and Fe65 Interaction
Design and Characterization of an Enhanced Repressor of Human Papillomavirus E2 Protein
Identifying novel, non-chelating inhibitors of MMP-9 using virtual screening, NMR spectroscopy, and x-ray crystallography
Efficient Computation of Configurational Binding Entropy and Free Energy Changes for Ligand Design
Neurosteroid binding domains of the NMDA receptor NR2B subunit
Next-generation Immunoprecipitation Kits Validated for Functional Applications
Kinetics of NF-kB “stripping” by IkBα
Three Hot-Spot Regions Define the High Affinity of Human Interleukin 15 for its Private α-Receptor
Efficient isolation and identification of intracellular protein complexes from mammalian cells using HaloTag technology
Adapting Phi-value analysis to the study of Intriniscally Disordered Protein binding
Identification of novel Beta-Catenin: TCF4 inhibitors through TR-FRET and cell based assays
Investigation of the binding between an asymmetric tetrameric chaperone SecB, preprotein and translocase SecA
Antioxidant effect of Schizandrae Fructus (SF) on human keratinocyte HaCaT cells
Effects of Cnidii Fructus, a medicinal herb, on Male Reproductive System in Mouse Testes
Initial events of the endocytosis for infectious bursal disease subviral particles
Mapping the Interface of an Ultra-low Affinity Receptor-ligand Interaction