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This study examined correlates and predictors of antitransgender prejudice (N = 298) in undergraduates at an urban university in the southeastern U.S. Right-wing authoritarianism, contact apprehension, and intergroup anxiety were strongly correlated with antitransgender prejudice. Contact with gay, lesbian, and transgender individuals negatively correlated with transgender prejudice. Hierarchical regression analysis revealed that contact apprehension, right-wing authoritarianism, and contact with gay men and lesbians were significant predictors of antitransgender prejudice. Implications for clinicians, educators, advocates, and other health providers are discussed.