The aim of the present study was to evaluate the validity and reliability of the Bangla version of the World Health Organization Quality of Life questionnaire (WHOQOL-BREF) in an adult population in Bangladesh. Approximately 200 adults in the Dhaka district were interviewed using a questionnaire containing the Bangla version of the WHOQOL-BREF, as well as questions related to sociodemographic data. To assess the reliability of WHOQOL-BREF, Cronbach's α was calculated, and test–retest reliability was evaluated using intraclass correlation coefficient (ICC) of the first and second administrations. For comparison, approximately 200 leprosy patients were also interviewed with the questionnaire to examine the discriminant validity between groups. On the whole, sufficient validity was observed, and the Bangla version of the WHOQOL-BREF was deemed to be valid and reliable in assessing the quality of life of an adult population in Bangladesh.