This study presented the development of a reliable and valid scale, the Discriminatory Attitudes Scale, for use in research related to discrimination in a Turkish context. First, related literature was searched and a pool of 69 items was generated to cover the basic forms of discrimination. Then, data were collected from 840 college students and subjected to exploratory factor analysis with varimax rotation, leaving 21 items. Next, a confirmatory factor analysis was performed, which confirmed a 4-factor structure. These factors were named Discrimination Against Homosexuals, Discrimination Against Women, Discrimination Based on Race or Ethnicity and Religion, and Discrimination Against Foreigners. Correlational analyses with ratings from the Left-Right Orientation Scale, Cronbach coefficient alpha, and expert opinion provided evidence on validity and internal consistency and reliability of the Discriminatory Attitudes Scale.