Despite abundant research on offspring of Holocaust survivors (OHS), it is relatively unknown how they function in middle age. Transgenerational effects of the Holocaust may be stronger among middle-aged OHS as they previously suffered from early inclement natal and postnatal environment and now face age-related decline. However, middle-aged OHS may successfully maintain the resilience they demonstrated at a younger age. This study performed a wide-spectrum functional assessment of middle-aged OHS and a comparison group (N = 364) drawn from the Israeli component of the Survey of Health, Ageing and Retirement in Europe (SHARE-Israel). OHS, and especially those with two survivor parents, reported a higher sense of well-being but more physical health problems than the comparison group. The discussion provides possible explanations for this mixed functional profile.