Harry, JR, Barker, LA, Mercer, JA, and Dufek, JS. Vertical and horizontal impact force comparison during jump landings with and without rotation in NCAA Division I male soccer players. J Strength Cond Res 31(7): 1780–1786, 2017—There is a wealth of research on impact force characteristics when landing from a jump. However, there are no data on impact forces during landing from a jump with an airborne rotation about the vertical axis. We examined impact force parameters in the vertical and horizontal axes during vertical jump (VJ) landings and VJ landings with a 180° rotation (VJR). Twenty-four Division I male soccer players performed 3 VJ and VJR landings on a dual-force platform system. Paired-samples t-tests (α = 0.05) compared differences in the first (F1) and second (F2) peak vertical ground reaction forces, times to F1 (tF1), F2 (tF2), and the end of the impact phase, vertical impulse, and anterior-posterior and medial-lateral force couples. Effect sizes (ES; large >0.8) were computed to determine the magnitude of the differences. Lower jump height (41.60 ± 4.03 cm, VJ landings; 39.40 ± 4.05 cm, VJR landings; p = 0.002; ES = 0.39), greater F2 (55.71 ± 11.95 N·kg−1, VJ; 68.16 ± 14.82 N·kg−1; p < 0.001; ES = 0.94), faster tF2 (0.057 ± 0.012 seconds, VJ; 0.047 ± 0.011 seconds, VJR; p = 0.001; ES = 0.89), greater anterior-posterior (0.06 ± 0.03 N·s·kg−1, VJ; 0.56 ± 0.15 N·s·kg−1, VJR; p < 0.001; ES = 1.83) and medial-lateral force couples (0.29 ± 0.11 N·s·kg−1, VJ; 0.56 ± 0.14 N·s·kg−1, VJR; p < 0.001; ES = 1.46) occurred during VJR landings. No other differences were identified. This kinetic analysis determined that landing from a jump with 180° airborne rotation is different than landing from a jump without an airborne rotation. Male Division I soccer players could benefit from increasing the volume of VJR landings during training to address the differences in jump height and force parameters compared with VJ landings.