Nursing is a physically active profession; however, nurses and nursing support staff may limit their physical activity (PA) on non-workdays. The purpose of this pilot study was to examine the effects of a 12-week pedometer-based PA intervention on daily steps of hospital nursing and support staff from two departments, emergency (ED) and oncology (ONC). The 12-week minimal-contact, self-managed intervention was designed to increase total and non-workday PA. Twenty participants with an average age of 47.6 ± 7.1 years completed the intervention. Significant differences were found between total daily steps at baseline (8,446) and at 12 weeks (10,655; p = .014), and between workday steps at baseline (10,398) and at 12 weeks (13,056; p = 0.005). Oncology unit participants significantly increased their non-workday steps from baseline (5,850) to 12 weeks (9,829; p = .003). As part of a comprehensive intervention, pedometers can promote total and non-workday PA of nursing and support staff.