Extensively hydrolyzed casein formula alone or with L. rhamnosus GG reduces β‐lactoglobulin sensitization in mice
Extensively hydrolyzed casein formula (EHCF) has been proposed for CMA prevention in at‐risk infants 1, and it is commonly used as a first‐line option for CMA treatment 2. The efficacy of extensively hydrolyzed formulas on food allergy prevention is still controversial 4. Conversely, in a prospective study of infants with confirmed CMA, treatment with EHCF induced faster tolerance acquisition compared with other available formulas (hydrolyzed rice formula, soy formula and amino acid‐based formula) 5. This effect was potentiated by the addition of the probiotic Lactobacillus rhamnosus GG (LGG) 5. Apart from being hypoallergenic, little is known regarding a possible immunomodulatory role of the casein hydrolysis‐derived peptides against CMA, whether alone or in combination with LGG. Immunomodulatory effects of EHCF have been demonstrated in a rat model of type 1 diabetes 8, suggesting possible similar effects against other immune‐mediated conditions, such as food allergy. Murine models have been adopted to investigate the immune mechanisms elicited by different dietary interventions in CMA 9. In this study, we investigated the mechanisms elicited by EHCF, either alone or in combination with LGG, against β‐lactoglobulin (BLG) sensitization in mice. Peptide fractions within EHCF were also characterized.