ANAQUIN: a software toolkit for the analysis of spike-in controls for next generation sequencing

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Abstract

Summary:

Spike-in controls are synthetic nucleic-acid sequences that are added to a user's sample and constitute internal standards for subsequent steps in the next generation sequencing workflow.

Summary:

The Anaquin software toolkit can be used to analyze the performance of spike-in controls at multiple steps during RNA sequencing or genome sequencing analysis, providing useful diagnostic statistics, data visualization and sample normalization.

Availability and Implementation:

The software is implemented in C ++/R and is freely available under BSD license. The source code is available from github.com/student-t/Anaquin, binaries and user manual from www.sequin.xyz/software and R package from bioconductor.org/packages/Anaquin

Contact:

anaquin@garvan.org.au or t.mercer@garvan.org.au

Supplementary information:

Supplementary data are available at Bioinformatics online.

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