ANAQUIN: a software toolkit for the analysis of spike-in controls for next generation sequencing

    loading  Checking for direct PDF access through Ovid



Spike-in controls are synthetic nucleic-acid sequences that are added to a user's sample and constitute internal standards for subsequent steps in the next generation sequencing workflow.


The Anaquin software toolkit can be used to analyze the performance of spike-in controls at multiple steps during RNA sequencing or genome sequencing analysis, providing useful diagnostic statistics, data visualization and sample normalization.

Availability and Implementation:

The software is implemented in C ++/R and is freely available under BSD license. The source code is available from, binaries and user manual from and R package from

Contact: or

Supplementary information:

Supplementary data are available at Bioinformatics online.

    loading  Loading Related Articles