Correlation study on chromogranin A genetic polymorphism and prognosis of critically ill patients☆,☆☆

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Abstract

Objective:

The objective was to investigate the correlation between single nucleotide polymorphism (SNP) of chromogranin A (CHGA) and prognosis of critically ill patients.

Methods:

We screened 357 critically ill patients consecutively admitted to our intensive care unit. The −89/−415/−462 SNP locus in the promoter region and the +9559/+9578/+9590/+9611 SNP locus in exon 7 coding of CHGA were genotyped by polymerase chain reaction and DNA sequencing technology. Subsequently, the correlation between genotype and prognosis of patients was analyzed.

Results:

(1) Three hundred critically ill Chinese Han patients were enrolled in the study. CHGA−415/−462/+9559/+9611 SNPs were polymorphically distributed. Phenotypes of the 4 SNPs were shown not to be in linkage disequilibrium, and there were no significant differences in the minor allele frequencies (MAFs) of the 4 SNPs between participants of this study and healthy people in Asia. (2) The CHGA−415 T/C MAF of the nonsurvival group was significantly higher than that of the survival group (MAF 0.3813 and 0.2864, respectively; P = .026). Survival analysis showed that there were significant differences between the CHGA−415 T/C mutation group (including TC and CC genotypes) and the wild-type group (TT genotype) (log rank = 8.887, P = .003). The mortality in the mutant group was significantly higher than that in the wild-type group (0.3333 and 0.1852, respectively; P = .004). (3) Binary logistic analysis showed that CHGA−415 T/C polymorphism was an independent risk factor for the mortality of critically ill patients (odds ratio, 2.286; 95% confidence interval, 1.165-4.484; P = .016).

Conclusions:

Critically ill patients with CHGA−415 T/C mutant genotype display higher 30-day mortality than those with the wild-type group. CHGA−415 T/C polymorphism is an independent risk factor of poor prognosis in critically ill Chinese Han patients.

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