Electrospun nanofibers are being utilized for a wide range of healthcare applications. A plethora of natural and synthetic polymers are exploited for their ability to be electrospun and replace the complex habitat provided by the extracellular matrix for the cells. The fabrication of nanofibers can be tuned to act as a multicarrier system to deliver drugs, growth factors and health supplements etc. in a sustained manner. Owing to its pliability, nanofibers reached its heights in tissue engineering and drug delivery applications. This review mainly focuses on various standardized parameters and optimized blending ratios for animal and plant proteins to yield fine, continuous nanofibers for effective utilization in various healthcare applications.