Parvovirus B19 infection modulates the levels of cytokines in the plasma of rheumatoid arthritis patients

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Abstract

Background

Parvovirus B19 (B19V) infection is associated with various autoimmune diseases. We investigated the levels of pro-inflammatory (IFNγ, TNFα, IL-2, IL-12) and anti-inflammatory (IL-4, IL-10) cytokines in the plasma of B19V DNA positive (B19+) and negative (B19−) rheumatoid arthritis (RA) patients in comparison with the control group (healthy persons).

Methods

Blood samples were collected from 118 patients with RA and 49 healthy voluntaries. B19V sequence was determined in whole blood and cell-free plasma DNA by nested PCR. The levels of cytokines in the plasma and cell culture medium from Concanavalin A (ConA) or B19V VP1 protein stimulated PBMC were determined by ELISA.

Results

The levels of IL-4, IL-10, IL-12, IL-2 and TNFα were higher in plasma of RA patients in comparison with control persons. B19+ controls and RA patients had lower levels of IFNγ in comparison with B19− controls and RA patients.

Results

Within RA patients the plasma levels of IFNγ were lower in patients with low RA disease activity or remission. Plasma level of IL-4 was increased and IL-10 level was decreased in B19+ RA patients in comparison with B19− RA patients and did not differ between B19+ and B19− controls. B19V infection did not affect plasma levels of IL-12, IL-2, and TNFα.

Results

ConA and B19 VP1 protein stimulated PBMC from RA patients produced less IFNγ than stimulated PBMC from the healthy controls.

Conclusions

B19V infection could differently modulate the amount of cytokines in the plasma of healthy persons and RA patients. Decreased production of IFNγ and raised level of plasma IL-4 in RA patients could lower antiviral clearance.

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