From Bench to Bedside: No Need to be Nervous About Microsuturing?
Historically, the prognosis for complete nerve injuries (either internal or external transections) has not been spectacular. Yes, direct repairs can sometimes restore nerve function rather dramatically, particularly for distal injuries in younger patients. However, proximal injuries, in mixed motor and sensory nerves, and/or involving “critical-sized” defects of 4 cm to 7 cm or longer, continue to be challenging, and the prognosis remains poor for most patients who experience these injuries.
Numerous advances in the last two decades have finally started to “move the needle” and improve the prognosis for the surgical management of peripheral nerve injuries. Viable off-the-shelf surgical conduits for short-segment gaps (or for overwrapping repair sites to limit scarring and axonal escape) have become widely available, and offer results comparable to direct repair for short-segment deficits, particularly for sensory nerves [4, 6]. Perhaps better still, decellularized human allograft nerve grafts (Avance, AxoGen, Inc, Alachua, FL, USA) have demonstrated meaningful sensory and motor recovery (M4-M5, representing near-normal to normal strength against resistance), potentially equivalent to autograft (or even direct repair ) without donor site limitations or morbidity, for defects as long as 5 cm [1, 6, 7]. Investigators have focused with renewed enthusiasm on the immunosuppressant FK506 (tacrolimus) based on better-than expected neurologic recovery in patients undergoing whole-hand transplants . Tacrolimus appears to enhance nerve regeneration through a noncalcineurin dependent pathway, and has been studied intermittently for decades for this purpose [12, 13]. Perhaps most exciting, we may finally be approaching the event horizon of the era of sutureless (or minimal-suture) nerve repair.
Why the need for minimal suture nerve repair? Microsuturing of nerves is fraught with difficulty, and can result in undesired complications such as scarring, fascicle ligation by the suture, or gapping and axonal escape resulting in a neuroma in continuity, particularly when not augmented with a nerve conduit, vein graft, or similar envelope. An alternative approach like fibrin glue has demonstrated equivalent or superior results to suture repair in numerous animal studies and one human study , but concerns about lack of tensile strength and secondary nerve gapping persist . Beyond fibrin glue, we have seen poly(ethylene glycol [PEG]) hydrogel improve resistance to gapping, show equivalence to suture, and demonstrate better results than fibrin sealant . The use of photochemical tissue bonding (PTB) represents an even-more-promising technique using a nonthermal, Nd/YAG laser, nonimmunogenic amnion-derived wrap, and a photoactive dye. Animal studies [5, 9] suggest improved electrophysiological outcomes, histologic recovery, and even gait compared to direct suture repair. A more-recent study  created a 15 mm defect in rat sciatic nerves and repaired the defect with reversed isografts. PTB repairs outperformed suture repair for both immediate and delayed (30 days) reconstruction with regard to muscle mass retention and histomorphometric recovery .