Many studies have reported that G-protein β3 subunit (GNB3) C825T polymorphism is associated with essential hypertension (EH), although this remains subject to debate. Thus, meta-analysis was carried out to clarify this relationship. A total of 75 articles, reporting 81 case-control studies evaluating 28 369 patients and 34 933 control individuals, were assessed. Overall, a significant association was observed in the dominant model (odds ratio (OR) = 1.11, 95% CI 1.04–1.19), recessive model (OR = 1.09, 95% CI 1.01–1.17), TT vs CC (OR = 1.16, 95% CI 1.05–1.28), CT vs CC (OR = 1.09, 95% CI 1.02–1.17), and additive model (OR = 1.07, 95% CI 1.02–1.13) after pooling all eligible studies. Subgroup analysis by ethnicity and gender demonstrated significantly increased EH only in Caucasians using the dominant (OR = 1.22, 95% CI 1.07–1.39; TT vs CC, OR = 1.29, 95% CI 1.07–1.54; CT vs CC, OR = 1.19, 95% CI 1.05–1.35) and additive (OR = 1.16, 95% CI 1.05–1.28) models. In summary, the present meta-analysis indicated the GNB3 C825T polymorphism is related to increased EH exclusively in Caucasians.