Apropos: “Gut Microbiota Differences in Children From Distinct Socioeconomic Levels Living in the Same Urban Area in Brazil”
In addition, the authors do not discuss the study's significant limitations, including the lack of a food diary collection, a crucial measure for establishing a baseline dietary picture for each study participant. Even though the authors discussed the differential dietary categories among the groups, it is curious that the authors decided not to do any kind of dietary data collection/analysis for this study group.
Finally, it is crucial, even in studies not evaluating microbiota composition dynamics over time, to collect multiple samples to effectively judge the accuracy and consistency of the sample collection process. These practices will reduce error and increase confidence in eventual study conclusions.
Future human gut microbiota–related studies must take greater care and deference in sound study design to avoid these problems in the future.