Dynamized ethyl alcohol improves immune response and behavior in murine infection withTrypanosoma cruzi

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Abstract

Aim

To evaluate the effects of dynamized ethyl alcohol (Ethylicum)6cH and 30cH in mice infected with T. cruzi.

Methods

In a blind, randomized and controlled assay, 63 eight-week-old, Swiss, male mice, infected with IP (1400 trypomastigotes, T. cruzi-Y-strain), were allocated into groups: CNI-non-infected (n = 12), CI-infected and non-treated (n = 17), Et6cH-infected, treated with Ethylicum 6cH (dilution 1:1012) (n = 17), Et30cH-infected, treated with Ethylicum 30cH (dilution 1:1060) (n = 17).

Methods

Treatment was administered 48 h before and after infection, followed by 56 h/56 h periods, until the 9th day after infection (a.i), for 16 h. Survival and mortality were assessed until the 82nd day after infection (a.i.). TNF-α, IL-6, IL-10, IL-5 and IL-17A cytokines were assessed in serum (3–4 animals/group), at time T0 (before infection), T8 and T12 (8th and 12th a.i), using the Mouse Cytokine 20-Plex Panel Magnetic Kit (Invitrogen, USA). Inflammation was determined in heart sections (eosin-hematoxylin staining) and behavior was analyzed with ANY-maze® software. The study was approved by the Animal Ethics Committee/UEM. Statistica 8.0 and R 3.0.2 software were used for statistical analyses.

Results

The greater survival observed in the Et6cH group was related to decreased inflammation in heart tissue and increased IL-5 at T0 (p < 0.05) and IL-10 at T8 (p < 0.05), characterizing the Th2 response. It was also related to shorter periods of immobility, observed on day 12 a.i. The higher mortality in the Et30cH group was related to increased inflammation in the heart and a higher concentration of IL-6 and TNF-α cytokines, characterizing the Th1 response.

Conclusion

The results demonstrate the beneficial effect of Ethylicum 6cH in acute murine infection by T. cruzi.

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