Isolation and characterization of a novel deoxynivalenol-transforming strain Paradevosia shaoguanensis DDB001 from wheat field soil

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Abstract

A Strain DDB001 having an ability to remove deoxynivalenol (DON) was successfully isolated from wheat field soil. On the basis of phenotypic, genotypic and phylogenetic data, the strain DDB001 was identified as Paradevosia shaoguanensis. Strain DDB001 could grow well and thoroughly eliminate 200 mg l−1 of DON in complete growth medium, but it could not utilize DON as the sole carbon source for growth in mineral salt medium. Analysing DON transformation products by HPLC-MS assumed that the strain could transform DON into a less toxic stereoisomer, 3-epi-deoxynivalenol (3-epi-DON). In addition, it did not require preincubation with DON for the expression of DON-transforming activity. To the best of our knowledge, this is the first report showing that a Paradevosia strain has the capability of DON-transforming. Therefore, strain DDB001 can be considered as a useful tool in feed decontamination.

Significance and Impact of the Study

Deoxynivalenol (DON) is one of the most frequently found mycotoxins in all major cereal grains worldwide. A DON-transforming bacterium, Paradevosia shaoguanensis DDB001, was isolated from wheat field soil. This study is the first report on isolation of a member of the genus Paradevosia as a potent DON-transformation micro-organism. The isolated bacterium has the potential to be utilized for detoxification of DON-contaminated feed.

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