Three‐dimensional reconstruction with serial whole‐mount sections of oral tongue squamous cell carcinoma: A preliminary study

    loading  Checking for direct PDF access through Ovid


Oral tongue squamous cell carcinoma (OTSCC) is the most common form of oral squamous cell carcinoma (OSCC). Many factors influence the prognosis of OTSCC, among which margin status is a crucial one.1 Despite an overwhelming consensus that failure to eradicate tumor at the primary site is the single largest cause of death for OSCC patients, the present method to access margin status is limited with small samples of limited area.3
Margin design is more difficult in deep connective tissue planes as compared to mucosal margins.3 A clear margin in OTSCC is often described as margins >5 mm away from the invasion front of tumor.4 To better understand the invasion pattern of OTSCC in a more comprehensive way, and to study the relation between invasion front and surgical margin, can provide useful information in margin design.
Invasion pattern has been found to correlate with the prognosis of OTSCC.5 In 2D observation, pattern of invasion (POI) is examined at the host/tumor interface according to the POI types 1 through 5 previously defined by Bryne et al6 The highest score of POI presented is taken as the worst pattern of invasion (WPOI), no matter how focal. WPOI is a significant histopathological factor for OSCC prognosis.5 Even though WPOI system is a convenient and valuable assessment in invasion pattern, it has its limitations because of the limited volume of sample. Therefore, 3D investigation is a promising tool for adding up to our knowledge of tumor invasion pattern, and this knowledge can be further applied in margin design.
As a first step, we aim to develop a method to generate a 3D reconstruction with serial whole‐mount sections of OTSCC, thus accurate accession could be possible. Detailed experience is shared in this article.
    loading  Loading Related Articles