ABCA1 affects placental function via trophoblast and macrophage

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Abstract

Aims:

To study the potential impact of ABCA1 on the function of the placenta.

Main methods:

Trophoblasts and macrophages were isolated from the placenta with enzymatic digestion; Immunofluorescence assay was used to detect the location of ABCA1 in cells; RT-PCR and Western-blot were used to detect the expression of ABCA1; The cholesterol efflux assays of primary trophoblasts was detected by Amplex Red cholesterol assay kit (Invitrogen);Inflammatory factor secretion from primary macrophages was detected by Elisa.

Key findings:

ABCA1 was mainly located on trophoblast membranes. Decreased ABCA1 expression in trophoblasts reduced the cholesterol efflux of trophoblasts (P < 0.01). while increased ABCA1 expression in trophoblasts reduced the cholesterol efflux of trophoblasts (P < 0.05). ABCA1 was uniformly expressed on the cell membrane, cytoplasm, and nucleus of macrophages. Decreased ABCA1 expression in macrophages, increased inflammatory factors but reduced IL-10 (P < 0.01). While increased ABCA1 expression in macrophages, reduced inflammatory factors but increased IL-10 (P < 0.01).

Significance:

ABCA1 may be a potential target for the prevention of gestation diseases.

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