In this study, we sequenced and characterized an interferon-stimulated gene Viperin homologue, LcViperin, from large yellow croaker (Larimichthys crocea). The LcViperin encodes 354 amino acids and contains an N-terminal amphipathic α-helix domain, a radical S-adenosyl-l-methionine (SAM) domain and a highly conserved C-terminal domain. The analyses of LcViperin promoter region revealed nine kinds of putative transcriptional factor binding sites, including five putative ICSBP (IRF-8) binding sites and one putative IRF-1 binding site, indicating that the expression of LcViperin might be induced by the type I IFN response. Phylogenetic analyses based on amino acid sequences showed that the Viperin of large yellow croaker is clustered together with its counterparts from other teleost fishes. The Real-time PCR analyses showed that the LcViperin was found to be ubiquitously expressed in ten examined tissues in large yellow croaker, with predominant expression in peripheral blood, followed by heart and gill. Expression analyses showed that the LcViperin was rapidly and significantly upregulated in vivo after poly (I:C) challenge in peripheral blood, head kidney, spleen and liver tissues. The results indicate that the LcViperin might play a pivotal role in antiviral immune responses.