The purposes of this study were to identify the level of fatigue and to examine the predictors of fatigue in female patients on hemodialysis. The subjects for this study were 130 female patients at one hemodialysis unit in northern Taiwan. On univariate analyses, patients with higher levels of fatigue were more likely to be older, and have less education, lower serum albumin levels, sleep disturbances, and perceived higher level of hemodialysis stressors. The findings of this study can help nephrology nurses recognize fatigue as an important nursing issue for female patients on hemodialysis. It is necessary to provide support, information, and alternative solutions for improving their fatigue.