Ultrasound-guided corticosteroid injection into the carpal tunnel is widely performed for the management of carpal tunnel syndrome (CTS). However, this injection may worsen the condition in patients with type 2 diabetes mellitus (DM). Polydeoxyribonucleotide (PDRN) was recently recognized as a substitute for corticosteroid due to its anti-inflammatory effects. A 41-year-old woman with type 2 DM was admitted to our rehabilitation clinic with a 1-month history of numbness and tingling in her right hand and first 3 fingers tips. She declined corticosteroid treatment because of a previous episode of uncontrolled blood glucose levels following corticosteroid injection. Therefore, ultrasound-guided PDRN injections into the carpal tunnel were carried out. At the 6-month follow-up, the patient demonstrated an improvement in the CTS symptoms without any complications. Thus, PDRN injections into the carpal tunnel can be an alternative to corticosteroid injections for CTS if there are limitations due to side effects of the latter.