AIDS. 19(9):887–896, JUNE 10TH, 2005

DOI: 10.1097/01.aids.0000171402.00372.c2

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PMID: 15905669

Issn Print: 0269-9370

Publication Date: June 10th, 2005


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Clonal breadth of the HIV-1-specific T-cell receptor repertoire in vivo as determined by subtractive analysis

M Killian;Rachel Sabado;Stephanie Kilpatrick;Mary Hausner;Beth Jamieson;Otto Yang;

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Abstract

Objective:Although the epitopic breadth of HIV-1-specific CD8 T lymphocyte (CTL) responses has been described, the T cell receptor (TCR) diversity of virus-specific cells remains poorly defined.Design and methods:To address this issue, we applied a novel technique for subtractive analysis of the HIV-1-specific CTL repertoire, combining specific deletion of peptide-specific cells by 5-fluorouracil with TCR spectratyping to identify clonal breadth of CTL recognizing individual peptides.Results:Comprehensive analysis of an infected individual reveals that nine identified HIV-1-specific responses are comprised of at least 38 distinct T-cell clones (ranging from two to 10 distinct clones per epitope).Conclusion:Given the potentially crucial role of T-cell receptor breadth for viral recognition and avoidance of escape, this subepitopic analysis of CTL may offer an important measure of cellular immunity for pathogenesis and vaccine studies.

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