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Esco, MR, Snarr, RL, Leatherwood, MD, Chamberlain, NA, Redding, ML, Flatt, AA, Moon, JR, and Williford, HN. Comparison of total and segmental body composition using DXA and multifrequency bioimpedance in collegiate female athletes. J Strength Cond Res 29(4): 918–925, 2015—The purpose of this investigation was to determine the agreement between multifrequency bioelectrical impedance analysis (BIA) and dual-energy x-ray absorptiometry (DXA) for measuring body fat percentage (BF%), fat-free mass (FFM), and total body and segmental lean soft tissue (LST) in collegiate female athletes. Forty-five female athletes (age = 21.2 ± 2.0 years, height = 166.1 ± 7.1 cm, weight = 62.6 ± 9.9 kg) participated in this study. Variables measured through BIA and DXA were as follows: BF%, FFM, and LST of the arms (ARMSLST), the legs (LEGSLST), the trunk (TRUNKLST), and the total body (TOTALLST). Compared with the DXA, the InBody 720 provided significantly lower values for BF% (−3.3%, p < 0.001) and significantly higher values for FFM (2.1 kg, p < 0.001) with limits of agreement (1.96 SD of the mean difference) of ±5.6% for BF% and ±3.7 kg for FFM. No significant differences (p < 0.008) existed between the 2 devices (InBody 720—DXA) for ARMSLST (0.05 kg), TRUNKLST (0.14 kg), LEGSLST (−0.4 kg), and TOTALLST (−0.21 kg). The limits of agreement were ±0.79 kg for ARMSLST, ±2.62 kg for LEGSLST, ±3.18 kg for TRUNKLST, and ±4.23 kg for TOTALLST. This study found discrepancies in BF% and FFM between the 2 devices. However, the InBody 720 and DXA appeared to provide excellent agreement for measuring total body and segmental LST. Therefore, the InBody 720 may be a rapid noninvasive method to assess LST in female athletes when DXA is not available.