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This study investigated between- and within-person associations among mean levels and variability in affect, diabetes self-care behaviors, and continuously monitored glucose in Latinos with type 2 diabetes.Fifty participants (M [SD] age = 57.8 [11.7] years, 74% women, mean [SD] glycosylated hemoglobin A1c = 8.3% [1.5%]) wore a “blinded” continuous glucose monitor for 7 days, and they responded to twice daily automated phone surveys regarding positive affect, negative affect, and self-care behaviors.Higher mean levels of NA were associated with higher mean glucose (r = .30), greater percent hyperglycemia (r = .34) and greater percentage of out-of-range glucose (r = .34). Higher NA variability was also related to higher mean glucose (r = .34), greater percent of hyperglycemia (r = .44) and greater percentage of out-of-range glucose (r = .43). Higher positive affect variability was related to lower percentage of hypoglycemia (r = −.33). Higher mean levels of self-care behaviors were related to lower glucose variability (r = −.35). Finally, higher self-care behavior variability was related to greater percentage of hyperglycemia (r = .31) and greater percentage of out-of-range glucose (r = −.28). In multilevel regression models, within-person increases from mean levels of self-care were associated with lower mean levels of glucose (b = −7.4, 95% confidence interval [CI] = −12.8 to −1.9), lower percentage of hyperglycemia (b = −0.04, 95% CI = −0.07 to −0.01), and higher percentage of hypoglycemia (b = 0.02, 95% CI = 0.01 to 0.03) in the subsequent 10-hour period.Near-to-real time sampling documented associations of glucose with affect and diabetes self-care that are not detectable with traditional measures.