The activity concentration of 226Ra (radium), 232Th (thorium) and 40K (potassium) has been measured in the soil samples collected from Mansa and Muktsar districts of Punjab (India) using NaI (Tikl) gamma detector. The concentration of three radionuclides (226Ra, 232Th and 40K) in the studied area has been varied from 18±4 to 46±5, 53±7 to 98±8 and 248±54 to 756±110 Bq kg−1, respectively. Radium equivalent activities (Raeq) have been calculated in soil samples for the assessment of the radiation hazards arising due to the use of these soil samples. The absorbed dose rate of 226Ra, 232Th and 40K in studied area has been varied from 8 to 21, 33 to 61 and 9 to 25 nGy h−1, respectively. The corresponding indoor and outdoor annual effective dose in studied area was 0.38 and 0.09 mSv, respectively. The external and internal hazard has been also calculated for the assessment of radiation hazards in the studied area.