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The viscosity of subretinal fluid is an important factor in transmitting force created by subretinal fluid motion to the retina. The variation of viscosity of subretinal fluid can explain variations in the effect of subretinal fluid motion on the retina. Pseudoplastic variation of subretinal fluid viscosity was confirmed using a cone-plate microviscometer. To explain the variability in viscosity between subretinal fluid specimens, 14 clinical variables associated with each of 46 specimens were examined. The strongest statistical relationship was between aphakia and lower viscosity.