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Enterococcus have emerged as antibiotic resistant in nosocomial infections, food pollutant and also found in root canal teeth. The presence of virulence factors leads to spreading and survive in a hospital or other environments. Different molecular studies have reported the importance of Enterococcus with putative virulence factors at infections. The host immune process is able to identify Enterococcus, although these virulence factors provided conditions to evasion from the immune system. Therefore, these determinants are the best candidates for new therapies strategy and controlling this problematic microorganism.