Structure of carbohydrate chains of fucolectin from the bark of golden rain shrubLaburnum anagyroides

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Abstract

Abstract—

A reductive LiBH4-ButOH cleavage of N-glycosylamide carbohydrate-peptide bond allowed splitting off of oligosaccharide chains of the fucolectin, the bark agglutinin from the shrub golden rain Laburnum anagyroides (LABA). Four N-glycans were isolated by HPLC, and their structures were elucidated by monosaccharide analysis and 1H NMR (500 MHz) spectroscopy: Man2Fuc1Xyl1GlcNAc2 (M2FX), Man3Xyl1GlcNAc2 (M3X), Man3Fuc1Xyl1GlcNAc2 (M3FX), and Man3Xyl1Fuc1GlcNAc3 (NM3FX). All the N-glycans contain D-xylose and three of them, L-fucose; they were found to be in a 1:8:3:1 ratio.

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