Secondary metabolites (SM) products permanently played an important role in medicine; fungi metabolites have increasingly become major players in recent pharmaceutical discovery in particular antimicrobial agents. The aim of study was to prepare and characterize of potential biological extract of culture filtrate of Trichophyton rubrum and tested as antibacterial, antioxidant agents. Local isolates of a dermatophyte T. rubrum were used and examined for production of SM. Extracts were checked for physical and chemical characterization using Fourier transform infrared spectrophotomete and HPLC and their cytotoxicity using a sensitive in-vitro brine shrimp lethality bioassay. The results revealed the isolation, preparation and characterization of different components of SM, included especially kojic acid, fusidic acid, amides and sulfones which gave antibacterial and antioxidant properties. In conclusion, the current study established the ability of a dermatophyte, T. rubrum to produce antibiotic-like substances, especially the sulfones which not isolated and recorded previously from T. rubrum.