Evaluation of the genotoxic potential of standardized extract ofGlycyrrhiza glabra(GutGard™)

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★ Glycyrrhiza glabra is widespread throughout the world for various therapeutic applications. ★ GutGard™, a standardized flavonoid rich de-glycyrrhizinated extract of G. glabra root. ★ GutGard™ was evaluated for possible mutagenicity using standard battery of genotoxicity tests. ★ GutGard™ did not exhibit mutagenic effect in TA98 and TAMix strains of Salmonella typhimurium. ★ GutGard™ did not increase the chromosomal aberrations or micronucleus formation in CHO-K1 cells.

Glycyrrhiza glabra Linn. (licorice) is widespread throughout the Mediterranean region and certain areas of Asia. Historically, the dried rhizome and root of the plant were used by the Chinese, Egyptian, Greek, Indian, and Roman civilizations as expectorant and carminative. In the modern medicinal system, licorice is used to treat liver ailments, dyspepsia, bronchitis, rheumatoid arthritis etc. Despite the extensive pharmacological applications, the genotoxic potential of G. glabra extract (GutGard™) has not been evaluated. Hence, this study was conducted to investigate the genotoxic potential of GutGard™ using battery of in vitro test systems: bacterial reverse mutation test (Ames II™), chromosome aberration (CA) and micronucleus (MN) tests. GutGard™ did not show significant increase in number of revertant colonies in Salmonella typhimurium strains (TA98 and TAMix) with/without S9 fraction. In CA and MN studies, GutGard™ did not show clastogenic effect at 4 and 18 h treatments with and without S9 fraction. Results indicated that GutGard™ is not mutagenic in a battery of genotoxicity tests.

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