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The possibility to assess the growth-retarding activity of triazole compounds, such as paclobutrazole, uniconazole, and azovite, by an express bioassay based on the evaluation of the α-amylase activity, which hydrolyzes starch into soluble sugars in the aleurone layer of barley (Hordeum vulgare L.), was investigated. The retarding effects of triazoles, when evaluated by the inhibition of amylase activity, decreased in the following order: uniconazole > azovite > paclobutrazole ≈ chlorocholine chloride. Among barley cultivars, cv. No-sovskii 9 was the most sensitive to the tested triazole compounds. At the concentrations of 10−7 to 10−3 M, these chemicals manifested notable growth-retarding activity in the bioassays with the seedlings of long stemmed pea cv. Torsdag and spring barley, cvs. Nosovskii 9, Moskovskii 2, and Zazerskii 85. All these express bioassays were validated for assessing the triazole growth-retarding activity.