Generation of a Mouse Strain That Produces Immunoglobulin ``kappa'' Chains with Human Constant Regions

    loading  Checking for direct PDF access through Ovid

Abstract

Humanized antibodies are highly efficient as immunotherapeutic reagents and have many advantages over rodent antibodies.A mouse strain was generated by gene targeting to replace the mouse ``kappa'' light chain constant (C) region gene with the human C sub ``kappa'' gene. Mice homozygous for the replacement mutation (C sub ``kappa'' R) produced normal concentrations of serum antibodies, most of which carry chimeric ``kappa'' light chains, and mounted normal immune responses to hapten-protein conjugates. This technology provides a feasible option for the generation of high-affinity humanized antibodies by means of the powerful somatic hypermutation-selection mechanism.

Related Topics

    loading  Loading Related Articles