The on-demand emission of coherent and indistinguishable electrons by independent synchronized sources is a challenging task of quantum electronics, in particular regarding its application for quantum information processing. Using two independent on-demand electron sources, we triggered the emission of two single-electron wave packets at different inputs of an electronic beam splitter. Whereas classical particles would be randomly partitioned by the splitter, we observed two-particle interference resulting from quantum exchange. Both electrons, emitted in indistinguishable wave packets with synchronized arrival time on the splitter, exited in different outputs as recorded by the low-frequency current noise. The demonstration of two-electron interference provides the possibility of manipulating coherent and indistinguishable single-electron wave packets in quantum conductors.