This study examines how gender interacts with the extent of occupational and industry sex segregation to affect family-to-work conflict, work-to-family conflict, coworker support, and supportive work-family culture. Using a theoretical framework that highlights the negative ramifications of working in a sex-atypical occupation or industry, we hypothesized that men and women would be impacted differently by the percentage of women in an occupation or industry. The data (N = 2,810) are from the 2002 National Study of the Changing Workforce (a US sample). Findings suggest that gender interacts with the percentage of women in an industry in significantly predicting coworker support and supportive work–family culture. Gender also interacts with the percentage of women in an occupation in significantly predicting family-to-work conflict.