ALTERATION OF MONONUCLEAR CELL IMMUNE-ASSOCIATED ANTIGEN EXPRESSION, INTERLEUKIN-1 EXPRESSION, AND ANTIGEN PRESENTATION DURING INTRA-ABDOMINAL INFECTION

    loading  Checking for direct PDF access through Ovid

Abstract

ABSTRACT

Intact peritoneal macrophage (Mø) function is critical to successful localization of intra-abdominal infection. Peritoneal macrophage antigen presentation capacity (APC), interleukin-1 (IL-1) expression, and immune-associated (la) antigen expression and abscess formation were determined following cecal ligation and puncture. APC and IL-1 expression were measured by coculture with a T-helper cell clone and by measuring subsequent proliferation, la expression was determined in blood, peritoneal Mø, and splenocytes using anti-la monoclonal antibody stain and flow cytometric analysis. Significant reductions in both la expression and APC were found 1 and 4 days after CLP with no change in IL-1 expression. Muramyl dipeptide, which enhances Mø phagocytosis, partially abrogated the depression in antigen presentation but did not affect la expression. Peritoneal Mø la expression and APC, but not IL-1 expression, were depressed after experimental peritonitis. The recovery of Mø function by day 14 coincides with clinical recovery and abscess formation, and restoration of early Mø depression may improve outcome.

Related Topics

    loading  Loading Related Articles