The objective of this study was to determine the degree of plasma volume expansion by 0.9% NaCl in relation to the infused volume, in sepsis/systemic inflammatory response syndrome (SIRS), after a standardized hemorrhage, and in a normal condition.Design
Prospective, randomized animal study.Setting
The study was performed at a university hospital laboratory.Subjects
Thirty anesthetized adult male rats were included in the study.Interventions
The study was performed in three groups: a sepsis/SIRS group (the S group), in which sepsis/SIRS was induced by cecal ligation and incision; a hemorrhage group (the H group), in which the rats were left without intervention for 4 h and bled 8 mL/kg thereafter; and a group that was left without intervention (the N group). Then, 4 h after baseline, all three groups were given an infusion of 0.9% NaCl (32 mL/kg) for 15 min. Baseline was defined as the time point when the surgical preparation was finished.Measurements and Main Results
Plasma volumes were measured using 125I-albumin dilution technique at baseline, after 4 h, and 20 min after the end of infusion. The plasma volume–expanding effect 20 min after end of infusion was 0.6% ± 2.9% in the S group, 20% ± 6.4% in the H group, and 12% ± 11% in the N group, compared with just before start of infusion.Conclusions
The present study in rats showed that the plasma volume–expanding effect after an infusion of 0.9% NaCl was smaller in a septic/SIRS state than after hemorrhage and in a normal state. This indicates that the plasma volume–expanding effect of a crystalloid is dependent on pathophysiologic changes in sepsis/SIRS.