In the present study, we validated a culturally adapted stigma scale designed to assess stigma among African Americans living with HIV. We collected data on the scale using an audio computer assisted self-interview (ACASI) format. We validated the scale with a sample of 62 African American participants living with HIV. Findings demonstrated that stigma can be measured succinctly and effectively in a 14-item scale with 2 subscales measuring enacted and internalized stigma. We identified many advantages to using the scale, which demonstrated good psychometric properties when used with an audio computer assisted self-interview format and with an African American sample. We recommend this scale’s use in both clinical practice and research study of HIV-stigma reduction interventions with African American populations.