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The purpose of this study was to assess the efficacy and safety of intraoperative enteroscopy (IOE) in patients undergoing minimally invasive surgery.Twelve patients underwent minimally invasive surgery and IOE at Miyazaki University Hospital. Patients included 11 men and 1 woman. After extraction of the intestine via minilaparotomy, enterotomy was performed, and a sterilized enteroscope was inserted.Length of the skin incision was 5.7±0.2 cm (mean±standard error). Length of the small intestine observed enteroscopically was 334±19 cm. Distance from the ligament of Treitz to the orally observed jejunum was 11.8±3.6 cm. In 5 of 9 patients with Crohn disease, additional lesions were found by IOE that were not found by preoperative examination. One additional tumor was found in 1 patient with ileal tumor. Postoperative complications occurred in 2 patients.IOE is efficacious in patients undergoing minimally invasive surgery.