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Transvaginal cholecystectomy encourages researchers to develop new hybrid or pure techniques for natural orifice surgery. We present an approach that combines flexible endoscopes, rigid instruments, and percutaneous needles.A 26-year-old female patient with cholelithiasis underwent a single-port culdolaparoscopy cholecystectomy at “Hospital Regional Poza Rica” (Poza Rica, Mexico) on July 2008. The surgery was performed with only 1 transvaginal 16 mm in diameter by 32 cm in length port. The instruments included a gastroscope, laparoscopic 5 mm in diameter by 43 cm in length instruments, percutaneous reins, and hook needle.The patient was discharged 24 hours after surgery, which is customary for the hospital, without pain or visible scars.Single transvaginal port cholecystectomy using a gastroscope and laparoscopic 5 mm instruments in a parallel path is a feasible procedure in selected patients. This technique requires no abdominal ports assistance.